Prevalence Of Metallo-Î’-Lactamase Producing Pseudomonas Spp. (In Tertiary Care Hospital)
Prevalence Of Metallo-Î’-Lactamase Producing Pseudomonas Spp.
Keywords:
Carbapenem-imipenem, Metallo-β-lactamase, Pseudomonas sppAbstract
Background: Metallo-β-lactamase (MBL) mediated resistance to carbapenem is an emerging threat in Pseudomonas isolates. The aim of this study is to detect metallo-β-lactamase producing isolates of Pseudomonas spp. from various clinical samples from indoor patients in a teaching hospital. Materials and Methods: Total 900 bacterial strains were isolated from different clinical samples from indoor patients. The bacterial strains were isolated and identified as per the standard guidelines. Amongst them 100 isolates of Pseudomonas were taken for the present study. All pseudomonas isolates were subjected to antimicrobial susceptibility testing by Kirby-Bauer disc diffusion method (CLSIs). In all imipenem resistant isolates of Pseudomonas spp., MBL detection was carried out by Imipenem-EDTA combined-disc synergy test (CDST). Results: Out of 100 isolates of Pseudomonas, 44 (44%) were imipenem resistant. Of these 44 isolates, 30 were producing MBL enzyme. 30 MBL positive isolate included 12 (40%) from surgical wards, 10 (33.33%) from tuberculosis ward, 4 (13%) from medicine ward, 2 (7%) from paediatric ward, 1 (3%) from urology ward and 1 (3%) from neonatal ICU. All MBL positive strains were resistant to β-lactams, aminoglycosides and fluoroquinolones. Conclusion: Prevalence of MBL producing Pseudomonas spp. is 30%. The MBL producing Pseudomonas spp. isolates were multidrug resistant. It is important to identify MBL producing pseudomonas isolates in laboratory as may cause serious infections and may cause a nosocomial outbreak.
References
2. Cedric M, John P, Ivan R, Derek W, Rosamund W. Medical Microbiology: Hospital infection, Sterilization and Disinfection: Mosby Publisher, London, 1998: 481
3. Greenwood SP. Medical Microbiology: Pseudomonas and Non fermenters, Churchill Livingstone Publisher, U.K., 1997: 287.
4. Timothy RW, Mark AT, Laurent P and Patrice N- metalloβ-lactamase a quite before storm. Clin.Microbiol. Rev. 2005; 18 (2): 306-25.
5. Maltezou HC. Metallo-β-lactamases in Gram-negative bacteria: introducing the era of pan- resistance? Int J Antimicrob Agents. 2009 May;33(5):405
6. Picao RC, Andrade SS, Nicoletti AG, Campana EH, Moraes GC, Mendes RE,etal.Metallo-β-Lactamase Detection: Comparative Evaluation of Double Disk Synergy versus Combined Disk
Tests for IMP, GIM, SIM, SPM or VIM Producing Isolates. J Clin Microbiol. 2008; 46(6):2028-37.
7. Mary JF, William AC, Michael ND, George ME, David WH, Janet H. NCCLS Performance Standards for Antimicrobial Disk Susceptibility tests; Approved standard-7th edition, NCCLS Publishers, Wayne PA,M2-A7 (2000).
8. Yan JJ, Wu JJ, Tsai SH, Chuang CL. Comparision of double-disk, combined disk, methods for detecting metallo-betalactamase in gram-negative bacilli. Diagn Microbiol Infect Dis. 2004; 49(1):5-11.
9. Johann DD, Daniel BG, Laurent J, Jo-Ann M, Phillip L and Deirdre LC. Detection of Pseudomonas aeruginosa producing metallo beta-lactamase in a Large centralized laboratory. J Clin Microbiol.2005; 43(7): 3129-35.
10. Mathur P, Behera B, Sharma V, Das A, Kapil A. An evaluation of four different phenotypic techniques for detection of metallo-β-lactamase producing Pseudomonas aeruginosa. IJMM .2008; 26: 233-37.
11. Mehul SC, Tanuja BJ, Govind N, Neelam P , Jivraj D. A study of metallo-β-lactatamase producing pseudomonas aeruginosa in clinical samples of S.S.G. hospital .NJMR;2011; 1 :60-3
12. Bashir D, Manzoor AT, Bashir AF, Gulnaz B, Danish Z, Shabir A at al. Detection of metallo-beta-lactamase (MBL) producing Pseudomonas aeruginosa at a tertiary care hospital in Kashmir. African Journal of Microbiology Research.2011; 5(2):164-72.
13. Hemlatha V, Sekar U, Kamat V- Detection of metallo-betalactamase producing Pseudomonas aeruginosa in hospitalized patients. Indian J. Med. Res.2005; 122: 148-52.
