An Invitro Study Of Determination Of Anti-Bacterial, Antioxidant, Anti- Inflammatory Potential Of Piper Betel Essential Oil
Piper betel essential oil.
DOI:
https://doi.org/10.70284/njirm.v6i2.878Keywords:
Antibacterial, Antioxidant, Anti-inflammatory, Piper betel essential oilAbstract
Background: Piper betel Linn is considered to possess important medicinal values. Leaves are considered more valuable part and was used in past for preventing halitosis. Essential oil obtained from leaves have been tried for antibacterial potential against various oral bacteria such as Streptococcous mutans, Actinomyces species and has demonstrated effective role in suppressing plaque formation. In addition essential oil obtained from leaves also exhibits anticancer properties because of antioxidant and anti-inflammatory potential. However available literature falls short in evaluating antibacterial potential of Piper betel essential oil against common periodontal pathogens- Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia. Present invitro study was conducted to evaluate antibacterial potential of Piper betel essential oil against previously mentioned periodontal pathogens by determination of minimum inhibitory concentrations (M.I.C).Antioxidant and Anti-inflammatory potential was also determined for the same. Methodology: Antibacterial potential was determined using Disc diffusion test and Broth Microdilution method. Antioxidant and anti-inflammatory potential was determined by measurement of superoxide dismutase (SOD) activity using riboflavin-NBT assay and detection of MMP-2 and MMP-9 by gelatin zymography method respectively. Results: Piper betel essential oil is an effective antibacterial activity against the tested periodontal pathogens along with antioxidant and anti-inflammatory potential. Conclusion: Piper betel essential oil possesses effective antibacterial, antioxidant and anti-inflammatory potential and could be used effectively in formulation of oral health care product. [Uppoor A NJIRM 2015; 6(2): 37-44]
References
2. Ramamurthy J, Lakshmi T.Pharmacological Aspects Of Tea Tree Oil (Tto) And Its Role In Dentistry- A Comprehensive Review. Int J Pharma. Bio. Sci.2011;2(4):572-581.
3. Zain NBM. Differential Expression of Gene of Streptococcus Mutans in Response to Treatment with Piper Betle Aqueous Extract – A Research Framework- 2011.International Conference on Bioscience, Biochemistry and Bioinformatics IPCBEE 5:467-469;2011.
4. Yanagida A.,Kanda T, Tanabe M, Matsudaira F, Cordeiro JGO. Inhibitory effects of apple polyphenols and related compounds on cariogenic factors of mutants streptococci. J. Agric. Food Chem 2000.48:5666–5671.
5. Sharma S, Khan IA, Ali I, Ali F, Kumar M, Kumar A,et al. Evaluation of the Antimicrobial, Antioxidant, and Anti-Inflammatory Activities of Hydroxychavicol for Its Potential Use as an Oral Care Agent. Antimicrob Agents Chemother2009; 53(1):216–222.
6. Rai MP, Thilakchand KR, Palatty PL, Rao P, Rao S, Bhat HP, et al. Piper Betel Linn (Betel Vine), the Maligned Southeast Asian Medicinal Plant Possesses Cancer Preventive Effects: Time to Reconsider the Wronged Opinion. Asian Pacific Journal Cancer Prev.2011;12: 2149-2156.
7. Wang CK, Chen SL, Wu MG. Inhibitory effect of betel quid on the volatility of methyl mercaptan. J. Agric. Food Chem. 2001;49: 1979–1983.
8. Ramji N, Ramji N, Iyer R, Chandrasekaran S. Phenolic antibacterials from Piper betel in the prevention of halitosis. J. Ethnopharmacol. 2002; 83:149-152.
9. Kaveti B, Tan L, Sarnnia, Kuan TS, Baig M. Antibacterial Activity Of Piper Betel Leaves.
International Journal of Pharmacy Teaching & Practices. 2011; 2(1):129-132.
10. National Cancer Institute. Chemical composition of smokeless tobacco products. In: Smokeless Tobacco or Health. An International Perspective (Smoking and Tobacco Control Monograph No.2), Bethesda (MD): Brunnemann KD, Hoffmann D; 1992.
11. Boffetta P, Hecht S, Gray N, Gupta P, Straif K. Smokeless tobacco and cancer. Lancet Oncol 2008; 9:667–75.
12. Sundqvist K, Liu Y, Nair J, et al. Cytotoxic and genotoxic effects of areca nut-related compounds in cultured human buccal epithelial cells. Cancer Research 1989; 49: 5294-8.
13. Jeng JH, Kuo ML, Hahn LJ, Kuo MY Genotoxic and non-genotoxic effects of betel quid ingredients on oral mucosal fibroblasts in vitro. JDent Res1994; 73:1043-9.
14. Thomas SJ, MacLennan R. Slaked lime and betel nut cancer in Papua New Guinea. Lancet.1992; 340: 577-8.
15. Bhide SV, Shivapurkar NM, Gothoskar SV, Ranadive KJ. Carcinogenicity of betel quid ingredients: feeding mice with aqueous extract and the polyphenol fraction of betel nut. British Journal of Cancer.1979; 40:922-6.
16. Razak FA, Rahim ZHA. The anti-adherence effect of Piper betle and Psidium guajava extracts on the adhesion of early settlers in dental plaqueto saliva-coated glass surfaces. Journal of Oral Science. 2003; 45(4):201-206.
17. Palombo EA. Traditional Medicinal Plant Extracts and Natural Products with Activity against Oral Bacteria: Potential Application in the Prevention and Treatment of Oral Diseases. Review article.Evidence-Based Complementary and Alternative Medicine.2011;1-16
18. Lai LS, Chang PC, Chang CT. Isolation and characterization of superoxide dismutase from wheat seedlings. J. Agric. Food Chem.2008; 56(17):8121–8129.
19. Chakraborty D, Shah B.Antimicrobial, Antioxidative and Antihemolytic Activity Of Piper Betel Leaf Extracts.International Journal of Pharmacy and Pharmaceutical Sciences (Suppl 3).2011; 192- 199.
20. Pradhan1 D, Suri KA, Pradhan DK, Biswasroy P. Golden Heart of the Nature: Piper betle L.Journal of Pharmacognosy and Phytochemistry.2013; 1(6):147-163.
21. Nair SS, Nithyakala CM, Rozario RV, Jennifer J, Somashekharaiah BV. Biochemical Characterization of Selected Plant Species and Investigation of Phytochemicals for In-Vitro Antioxidant Activity. International Journal of Pharmacognosy and Phytochemical Research. 2012; 4(3):127-13.
22. Makpol S, Yeoh TW, Ruslam FAC, Arifin KT, YusofYAM. Comparative effect of Piper betle, Chlorella vulgaris and tocotrienol-rich fraction on antioxidant enzymes activity in cellular ageing of human diploid fibroblasts. BMC Complementary and Alternative Medicine. 2013; 13:210.
23. Beauchamp C, Fridovich I. Superoxide Dismutase:Improved Assays and an Assay Applicable to Acrylamide Gels. Analytical Biochemistry.1971; 44:276-287.
