Isolation Of Beta Lactamases Engendering Lactose Fermenters From Different Categories Of Neonatal Sepsis Cases: Isolation Of Beta Lactamases Engendering Lactose Fermenters

Prem Prakash Mishra; Dakshina Bisht; Ved Prakash Sharma; Varun Goel

doi:10.70284/njirm.v10i2.2523

Authors

Prem Prakash Mishra Phd Scholar, Department of Microbiology, Santosh Medical College & Hospital, (Santosh Deemed to be University, NCR Delhi), Ghaziabad, NCR, U.P, India
Dakshina Bisht Assistant Professor, Department of Microbiology, Santosh Medical College & Hospital, (Santosh Deemed to be University, NCR Delhi), Ghaziabad, NCR, U.P, India
Ved Prakash Sharma Professor & Head , Department of Microbiology, Rohilkhand Medical College & Hospital, Bareilly, U.P, India, 243006
Varun Goel Assistant Professor, Department of Microbiology, Santosh Medical College & Hospital, (Santosh Deemed to be University, NCR Delhi), Ghaziabad, NCR, U.P, India

DOI:

https://doi.org/10.70284/njirm.v10i2.2523

Keywords:

AmpC, ESBL, E.coli, Klebsiella, Neonatal sepsis.

Abstract

Background: The escalating incidence of ESBLs and Amp C producers in various grievous clinical conditions has convoluted treatment strategies. The objective of this study is to determine the incidence of ESBLs and AmpC Î²-lactamase producing Escherichia coli and Klebsiella spp from different types of neonatal sepsis cases. Methodology: This cross sectional study included 382 blood samples which were processed by standard microbiological methods. A.S.T was carried out by Kirby Bauer disk diffusion method as per CLSI guidelines. The presumptive producers of ESBLs were confirmed as per CLSI guidelines (2015). Inducible AmpC were detected by Disc antagonism test (DAT) and E test. Results: The blood culture positivity was 32.46% (n=124/382) which was higher in males (34.36%) as compared to females (29.67%). Culture positivity among the EONS and LONS cases were found to be 47.38% and 52.42% respectively. There was dominance of Gram negative isolates (58.87%) over Gram positive isolates (37.9%) and Yeast like fungi (3.23%). E. coli (41.09%) was significantly isolated from EONS cases while Klebsiella spp (31.51%) was isolated almost dispassionately from both types. Among the 45.28% ESBL producing isolates, 58.33% (14) isolates of E.coli (09) and Klebsiella species (05) were recovered from EONS cases while 10 (41.67%) strains of E.coli (04) and Klebsiella species (06) were recuperated from LONS cases. The isolation of inducible AmpC was found to be 13.79%. Pure AmpC (both from EONS cases) as well as co-production of ESBL and AmpC (01 from LONS and 01from EONS cases) was seen in 01 each of E.coli and Klebsiella spp. Conclusion: The differentiation of ESBL from AmpC Î²-lactamases is necessary for formulation of treatment guidelines as the incidence is escalating.[Mishra P Natl J Integr Res Med, 2019; 10(2):29-34]

Isolation Of Beta Lactamases Engendering Lactose Fermenters From Different Categories Of Neonatal Sepsis Cases