Use of geneXpert in the diagnosis of paediatric tuberculosis – A retrospective study in Northern Maharashtra

Use of geneXpert in the diagnosis of paediatric tuberculosis – A retrospective study in Northern Maharashtra

Authors

  • Sunil Lilani Associate Professor, Dept. of Microbiology, Shri Bhausaheb Hire Govt. Medical College, Dhule, Maharashtra
  • Pooja Shah Tutor, Dept. of Microbiology, Govt. Medical College, Akola, Maharashtra
  • Madhuri Suryawanshi Assistant Professor, Dept. of Microbiology, Shri Bhausaheb Hire Govt. Medical College, Dhule, Maharashtra
  • M. N. Dravid Professor and Head, Dept. of Microbiology, Shri Bhausaheb Hire Govt. Medical College, Dhule, Maharashtra

DOI:

https://doi.org/10.70284/njirm.v9i5.2420

Keywords:

GeneXpert, MDR-TB, 2016-2017, Pediatric

Abstract

Introduction:Tuberculosis (TB) is an important cause of childhood morbidity and mortality. Prevalence of childhood TB has been reported to be between 3 and 25% in different countries. GeneXpert gives results in approximately 4 hours and hence it is widely used. Aims and objectives:
1. To study the occurrence of tuberculosis in pediatric patients.
2. To study the age and sex predominance of TB.
3. To study occurrence of MDR-TB(Multi Drug Resistant) in pediatric age group.
Material and Methods:Sample testing was done using GeneXpert system as per manufacturer’s guidelines from January 2016-December 2017. Results:Total 186 samples were suspected for pediatric TB from January 2016-December 2017. 11.48% samples were positive for TB by geneXpert. 9-12 years age group was most commonly affected(57.14%). Males were more affected than females(2:1). Sputum sample was most common sample obtained followed by gastric lavage and pleural fluid. MDR TB was seen in 14.29% cases. Conclusion: GeneXpert proves to be important method in detection of tuberculosis with early results.

References

1. Kabra SK, Lodha R, Seth V. Category based treatment of tuberculosis in children. Indian Pediatr 2004;41:927-37.
2. The Registrar General & Census Commissioner, India, New Delhi, Ministry of Home Affairs, Government of India.(online )2001. Available from: http://www.censusindia.gov.in/Census_Data_2001/India_at_Glance/broad.aspx.
3. Stop TB. Partnership childhood TB, subgroup world health organization: Guidance for national tuberculosis programmes on the management of tuberculosis in children. Chapter 1: Introduction and diagnosis of tuberculosis in children. Int J Tuberc Lung Dis 2006;10:1091-97.
4. Nelson LJ, Wells CD. Global epidemiology of childhood tuberculosis. Int J Tuberc Lung Dis 2004;8:636-47.
5. Kumar A, Gupta D, Nagaraja SB, Singh V, Sethi GR, et al. Updated national guidelines for pediatric tuberculosis in India. Indian Pediatr 2012;50:301-06.
6. Anne K Detjen, Andrew R DiNardo, Jacinta Leyden, Karen R Steingart, Dick Menzies. Xpert MTB/RIF assay for the diagnosis of pulmonary tuberculosis in children: a systematic review and meta-analysis. Lancet Respir Med 2015;3(6):451–61.
7. Walusimbi S, Bwanga F, De Costa A, Haile M, Joloba M, Hoffner S. Meta-analysis to compare the accuracy of GeneXpert, MODS and the WHO 2007 algorithm for diagnosis of smear-negative pulmonary tuberculosis. BMC Infectious Dis 2013;13:507.
8. Tuberculosis. WHO Global Tuberculosis Report 2014. http://www.who.int/ tb/ publications/factsheet global.pdf.
9. Weyer K, Mirzayev F, Migliori GB, Gemert WV, D’Ambrosio L, Zignol M. Rapid molecular TB diagnosis: evidence, policy making and global implementation of Xpert MTB/RIF. ERJ 2013;42:252–71.
10. Reena Raveendran, Jaswinder Kaur Oberoi & Chand Wattal. Multidrug-resistant pulmonary & extrapulmonary tuberculosis: A 13 years retrospective hospital-based analysis. Indian J Med Res 2015;142:575-82.
11. Mukherjee A, Chowdhury R, Singla R, Saha I, Dutta R, Das T. Comparison between childhood and adult tuberculosis in a rural tuberculosis unit of West Bengal: A retrospective study. Lung India 2014;31:116-20. 12. Verma D, Sharma R, Chand A.E, Saxena A. The application of gene xpert for the diagnosis of mycobacterium tuberculosis and MDR TB in Kota region. Int J Med Res Rev 2016;4(10):1863-70.
13. Gandra NR, Gali JH. GeneXpert: a game changer in the detection and diagnosis of childhood tuberculosis. Int J Contemp Pediatr 2018;5:35-41.
14. Iravane J.A., et al., (2017) GeneXpert for Gastric Lavage to Diagnose Pulmonary Tuberculosis in Children. Int J Microbiol Res 2017;9(11):966-69.
15. Husseiny Sh. Moussa, Faten S. Bayoumi, Ahmed M. Ali. Evaluation of GeneXpert MTB/RIF assay for direct diagnosis of pulmonary tuberculosis. Saudi Med J 2016;37(10),1076-81.
16. S Rama Prakasha, G Suresh, Ivor Peter D’sa, Shobha S Shetty,S Ganesh Kumar. Mapping the Pattern and Trends of Extrapulmonary Tuberculosis.J Glob Infect Dis 2013; 5(2):54–9.
17. Giang DC, Duong TN, Ninh HA DT, Nhan HT, Wolbers M, Nhu NTQ et al. Prospective evaluation of GeneXpert for the diagnosis of HIV-negative pediatric TB cases. BMC Infectious diseases 2015.
18. Azger Dusthackeer, Gomathi Sekar, Shambhavi Chidambaram, Vanaja Kumar, Pranav Mehta, Soumya Swaminathan. Drug resistance among extrapulmonary TB patients: Six years experience from a supranational reference laboratory.Indian J Med Res 2015;142:568-657.

Downloads

Published

2018-11-01

How to Cite

Lilani, S., Shah, P., Suryawanshi, M., & Dravid, M. N. (2018). Use of geneXpert in the diagnosis of paediatric tuberculosis – A retrospective study in Northern Maharashtra: Use of geneXpert in the diagnosis of paediatric tuberculosis – A retrospective study in Northern Maharashtra. National Journal of Integrated Research in Medicine, 9(5), 20–22. https://doi.org/10.70284/njirm.v9i5.2420

Issue

Section

Original Articles

Most read articles by the same author(s)