A Study of Anaerobic Organisms in Clinically Suspected Cases of Anaerobic Infections
A Study of Anaerobic Organisms
Keywords:
Bacteroids fragilis, Prevotella melaninogenicusAbstract
Background: Anaerobes are microorganisms grow in tissue with reduced oxidation-reduction potentials, predisposing to infection in absence of oxygen. Most of anaerobic infection occurs when a patient’s normal flora gains access to a sterile site as a result of disruption of some anatomical barrier. Moreover, most anaerobic infections involve a mixture of aerobic and anaerobic organisms, so failure of treatment occurs because of mixed infections with both type of bacteria. The aim of study was to isolate and identify anaerobic bacteria in clinically suspected pure anaerobic infection and mixed infection. Also to know antibiotic sensitivity pattern of anaerobes for better treatment of clinical failure cases. Method: Different samples for culture collected either by biopsy or by aspiration using sterile needle and syringe. Anaerobic transport medium was used for sample collected on swab. Liquid anaerobic medium like Thioglycolate medium, Robertson cooked meet medium and solid anaerobic media like Blood agar with antibiotics, hemin, Vit. K, Bacteroides bile esculin agar and Kanamycin- Vancomycin blood agar were used for cultivation. COMPACT TG Anaerobic work station was used for providing oxygen free atmosphere for growth of anaerobic bacteria. Anaerobes isolated in 68(62.96%) out of total 108 samples from different pyogenic lesions of patient admitted in various wards of Gujarat Cancer and Research Institute, Ahmedabad during April 1998 to Dec. 1999. Result: B. fragilis and P. melaninogenicus were commonest isolate 21(35%) and 23(38.33%) respectively. Metronidazole (93%) and clinamycin(73%) were effective drugs for anaerobic bacteria. 58 samples(53.70%) were positive for both aerobes and anaerobes indicating synergisms for infection between these bacteria.
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