Antibiotic Resistance Pattern of Urinary Tract Isolates of Escherichia coli from Kumaun Region
Antibiotic Resistance Pattern of UTI
Keywords:
E.Coli, UTI, Antibiotic ResistanceAbstract
Background: Concurrent resistance to antimicrobials of different structural classes has arisen in a multitude of bacterial species and may complicate the therapeutic management of infections, including those of the urinary tract. Aim: To assess the resistance pattern among Escherichia coli from urinary isolates a retrospective in vitro surveillance study carried out in the Department of Microbiology, Uttarakhand Forest Hospital Trust Medical College from Jan. 2008- Dec. 2008. Materials and methods: Urine specimens were transported in sterile, leak-proof containers to the bacteriology laboratory immediately or if there was a delay, refrigerated for 4hrs before processing. Specimens were inoculated on cystine lactose electrolyte-deficient medium by semi quantitative method. The specimen yielding more than or equal to 105 organisms/ml of urine was interpreted as significant. Bacterial count less than this were considered insignificant. Growth of 3 or more than 3 organisms were considered contamination. Bacterial pathogens were identified by conventional biochemical methods according to standard microbiological techniques. Antimicrobial sensitivity was performed by the standard disk diffusion method. Results: E.coli was the most common uropathogen (57.76%) isolated and 94.29% of E.coli showed resistance to ampicillin, 92% to amoxycillin- salbactam, 70.86% to gentamicin, 65.71% to amikacin, 89.71% to cefuroxime, 72.57% to cefotaxime,76% to cefriaxone, 90.28% to ciprofloxacin, 61.14% to chlormphenicol, 42.86% to nitrofurantoin, 28% to piperacillin-tazobactam and 8.57% to imipenem. Multidrug resistance was defined as resistance to two or more classes of antibiotics. Multidrug resistance was detected in 97% isolates. Conclusion: These findings call for wiser use of antimicrobial agents and their continuous in vitro monitoring.
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