Neonatal Septicemia: Bacterial Isolates & Their Antibiotics Susceptibility Patterns
Neonatal Septicemia
Keywords:
Neonatal septicemia, antibiotic sensitivity test, KlebsiellaAbstract
Background: Septicemia remains a significant cause of morbidity and mortality in the newborn, more so in developing countries
due to delivery and postnatal follow up in an unclean environment having more chance of contamination with infective organisms. Moreover
these infants are deficient in their inherent protective mechanisms, humoral and cellular immunity. The changing pattern and frequent
emergence of resistant bacteria make the problem more difficult. Objectives: (1) To know the etiology of septicemia in neonates. (2) To
detect the antibiotic susceptibility pattern of the isolates. Methods and Material: Blood samples were collected aseptically from 303 newborns
admitted in NICU, Sir T Hospital, Bhavnagar during Jan-2006 to August-2008 with sepsis. The specimens were inoculated into brain heart
infusion broth & subcultures were performed. The isolates were identified by standard biochemical tests. Antibiotic sensitivity pattern of
isolates was studied by Modified Kirby Bauer Disc diffusion technique. Results: A total 140 (46.20%) organisms were isolated. These included
Klebsiella (66, 47.14%), Staphylococcus aureus (35, 25%), Coagulase negative staphylococci (CONS) (5, 3.57%), E.coli (15, 10.71%), Proteus
(5, 3.57%), Acinetobactor (3, 2.14%), Pseudomonas (6, 4.28%) and Candida (5, 3.57%). Majority of organisms isolated were resistant to
commonly used antibiotics. Maximum sensitivity was seen by Cefoperazone/sulbactum (97%) & Piperacillin/tazobactum (98%) for Gram
negative organisms & Vancomycin(100%) for Gram positive organisms. Conclusions: Multi-drug resistance organisms were isolated from
septicemia in neonates. Therefore great caution is required in selection of antibiotic therapy.
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