Prevalence of Thalassemia and Hemoglobin Variants by HPLC in Out Patient Laboratory
Prevalence of Thalassemia and Hemoglobin Variants by HPLC
Keywords:
HPLC (high performance liquid chromatography), Hemoglobinopathies, Hb Variants, ThalassemiaAbstract
Background: Inherited disorders of hemoglobin are common and their identification is essential epidemiologically and help to prevent serious hemoglobin disorder. Method: study was conducted in patients attending outpatient laboratory, Civil Hospital, Ahmedabad. Total 466 cases were scrutinized on basis of Mentzer’s index (MCV /RBC count < 13) and sent for HPLC at Red Crosssociety, Ahmedabad. Result: The Out of these, abnormal hemoglobin fraction was found in 107(22.90%) cases. Beta thalassemia trait was found predominantly. Other Hb variants like HbS, HbE, Hb D Punjab, Hb F and some double heterozygous Hb variants like Hb S-α thalassemia, Hb D-β thalassemias were also observed. Conclusion and Interpretation: Beta thalassemia trait was found to be predominant. [Dhruti P NJIRM 2017; 8(2):23-27]
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