Evaluation Of The Effect Of Smoking On Select Cytomorphometric Indices Of Buccal Mucosal Cell In Middle Aged Individuals: Evaluation of the Effect of Smoking On Select Cytomorphometric Indices

Alka Meena; Jitendra Gupta; Kapil Gupta; Umesh Kumar; Sanjay Singhal; Amitabh Dube

Authors

Alka Meena
Jitendra Gupta
Kapil Gupta
Umesh Kumar
Sanjay Singhal
Amitabh Dube

Keywords:

Oral exfoliative cytology, Light microscopy, Smokers and Non Smokers

Abstract

Background: The aim of the present study was to evaluate the effect of smoking on select cytomorphometric indices of buccal mucosal cell in middle aged individuals. Selected measurements of cytomorphometric indices such as nuclear area (NA) (Î¼m2), nuclear perimeter (NP) (Î¼m), minimal nuclear diameter (Dmin) (Î¼m) and maximal nuclear diameter (Dmax) (Î¼m) were observed and assayed through light microscopy making use of digital image processing software TS view version 7.3.1.7. Methods: 60 smokers and an equal number of age and sex matched control in the age range of 40 - 50 years were recruited in the present study. On exfoliative buccal mucosal cytology average nuclear area (NA), nuclear perimeter (NP), minimal nuclear diameter (Dmin) and maximal nuclear diameter (Dmax) values of cell nuclei were measured using the software TS view version 7.3.1.7. Results: Statistically significant increased cytomorphometric mean values of nuclear i.e. measurements NDmax (Î¼m) (P=0.000), NDmin (Î¼m) (P=0.000), NA (Î¼m2) (P=0.000), and NP (Î¼m) (P=0.004) in smokers as compared to that seen in non-smokers. Conclusion: In the present study, statistically significant increase in the cytomorphometric nuclear parameters, namely minimum nuclear diameter (NDmin), maximum nuclear diameter (NDmax), nuclear area (NA) and nuclear perimeter (NP), could be appreciated in smokers as compared to that observed in non-smokers, underscoring the relevance of exfoliative cytology as a potential screening assay for the diagnosis of dysplastic changes in the oral mucosal cells. [Alka M NJIRM 2017; 8(1): 66-70]

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Evaluation Of The Effect Of Smoking On Select Cytomorphometric Indices Of Buccal Mucosal Cell In Middle Aged Individuals