To Evaluate The Antimicrobial Efficacy Of Green Coffee Bean Extract On Periopathogens- A Clinico-microbiological Study
To Evaluate The Antimicrobial Efficacy Of Green Coffee Bean Extract On Periopathogens
DOI:
https://doi.org/10.70284/njirm.v7i5.1141Keywords:
Chlorogenic acid, Green coffee bean extract, Minimum inhibitory concentration, Periopathogens, thioglycolate brothAbstract
Aim - To evaluate the Antibacterial efficacy of three different dilution of green coffee bean extract on periopathogens. Objective- To achieve a dilution of green coffee bean extract which has the maximum inhibitory effect on P. gingivalis and A. actinomycetocomitans. Methods- The sterilised blood agar culture plate was prepared on which colonies of P. gingivalis and A. actinomycetocomitans were cultured by subgingival sample taken from Chronic Generalized Periodontitis cases. Three dilution of green coffee bean extract were prepared i.e 10 -8, 10 -9 and 10 -10 with the serial dilution method using Distilled water. Then streaking of colonies was done on three different areas of culture plate on which different dilution was added. Minimum inhibitory concentration (MIC) on culture plates was observed to see the inhibitory effect of Green Coffee Bean extract on periopathogens by Agar Diffusion Method.Result- 10-9 was found have maximum inhibitory effect on periopathogens especially P.Gingivalis Conclusion- P.Gingivalis is more susceptible to 10-9 concentration of Green Coffee Bean Extract [Sachin M NJIRM 2016; 7(5):56-59]
References
2. AG Antonio, RS Moraes, D Perrone, LC Maia, KRN Santos, NLP Iorio, Species, roasting degree and decaffeination influence the antibacterial activity of coffee against Streptococcus mutans Food Chemistry 2010 ;118:782-88.
3. Fardiaz S. Antimicrobial activity of coffee (Coffea robusta) extract. ASEA Food J. 1995; 10:103–6.
4. Karunanidhi A, Thomas R, van Belkum A, Neela V. In vitro antibacterial and antibiofilm activities of chlorogenic acid against clinical isolates of Stenotrophomonas maltophiliaincluding the trimethoprim/sulfamethoxazole resistant strain.Biomed Res Int. 2013; 2013:3920-58.
5. Farah A, Monteiro M, Donangelo CM, Lafay S. Chlorogenic acids from green coffee extract are highly bioavailable in humans. J Nutr. 2008; 138:2309–15.
6. Schwalbe R, Steele-Moore L, Goodwin AC. Macro-and microdilution methods of antimicrobial susceptibility testing. In: Schwalbe R, editor. Antimicrobial Susceptibility Testing Protocols. Florida, Fla, USA: AC: CRC Press Taylor and Francis Group; 2007. pp. 76–9.
7. M Li, Z Xu, Quercetin in a Lotus Leaves Extract May be Responsible for Antibacterial Activity Arch Pharm Res 2008 31(5):640-44.
8. Y Miyake, M Hiramitsu, Isolation and extraction of antimicrobial substances against oral bacteria from lemon peel J Food Sci Technol 201148(5):635-39.
9. LE Wolinsky, S Mania, S Nachnani, S Ling, The inhibiting effect of aqueous Azadirachtaindica (Neem) extract upon bacterial propertiesinfluencing in vitro plaque formation. J Dent Res 1996 75(2):816
10. P Agarwal, L Nagesh, Comparative evaluation of efficacy of 0.2% Chlorhexidine, Listerine and Tulsi extract mouth rinses on salivary Streptococcus mutans count of high school children-RC Contemp Clin Trials 2011 32(6):802-08. 12. K Borhan
11. Mojabi, M Sharifi, T Karagah, Efficacy of different concentrations of garlic extract in reduction of oral salivary microorganisms Arch Iran Med 2012 15(2):99-101.
12. Brandao F, Oliveira L, Landucci L, Koga-Ito C. Antimicrobial activity of coffee-based solutions and their effects on Streptococcus mutans adherence. Braz J Oral Sci.2007; 6:1274–80
13. Pure green coffee. Where the secrets of green coffee bean extract is hiding? [Last updated on 2013 Aug 14; Last cited on 2013 Sep 19].
14. Pruthviraj P, Suchita B, Shital K, Shilpa K. Evaluation of antibacterial activity of caffeine. Int J Res Ayurveda Pharm.2011; 2:1354–7.
15. Brandao F, Oliveira L, Landucci L, Koga-Ito C. Antimicrobial activity of coffee-based solutions and their effects on Streptococcus mutans adherence. Braz J Oral Sci.2007; 6:1274.
16. Toda M, Okubo S, Hiyoshi R, Shimamurat T. The bactericidal activity of tea and coffee. Lett Appl Microbiol.1989; 8:123–5.
