Evaluation Of Efficacy Of 0.2% Chlorohexidine Mouthwash As A Pre-Procedural Rinse In Reduction Of Streptococcal Colonies Following Ultrasonic Scaling: A Pilot Study

Evaluation Of Efficacy Of 0.2% Chlorohexidine Mouthwash As A Pre-Procedural Rinse In Reduction Of Streptococcal Colonies Following Ultrasonic Scaling: A Pilot Study

Authors

  • Sachit Anand Arora
  • Shivjot Chhina
  • Divya Sahay
  • Gunjan Puri
  • Navneet Kaur
  • Deeksha Bhardwaj

Keywords:

Chlorohexidine, Aerosols, Pre-procedural mouth rinse

Abstract

Objectives: To evaluate the efficacy of chlorohexidine mouthrinse as a pre-procedural rinse in reducing streptococcal bacterial colonies.Study Design: A single-centre, double-masked, placebo-controlled randomised clinical trial conducted over a period of 15 days. 6 patients were included in the study, patients were first asked to rinse with the placebo (distilled water) for 1 minute before the scaling of control site followed by test site rinsing with chlorhexidine mouthwash for 1 minute. The microbial contamination was checked in the operatory, operator’s mouth mask, the patient’s chest, the operator’s chest and the patient’s breath with the help of agar plates. Results: The results revealed that there was statistically significant reduction in the Colony Forming Units (CFU's) formation. Paired sampled t test was used for checking the statistical significance, confidence interval (CI) was 95% and (p<0.05).There was statistical reduction in CFU's in breath sample, 30 minutes after scaling and less number of CFU’s were formed on the operator’s mask after the scaling of the patients with the use of chlorhexidine as the pre-procedural rinse compared to distilled water. Conclusion: The 0.2% chlorohexidine mouth rinse as a pre-procedural rinse has comparatively greater efficacy than distilled water in reduction of streptococcal colony forming units, however this finding was not statistically significant.[Sachit A NJIRM 2016; 7(5):46-52]

References

1. Lu DP, Zambito RF. Aerosols and cross infection in dental practice— a historic view. Gen Dent. 1981;29:136–143.
2. King TB, Muzzin KB, Berry CW, Anders LM. The effectiveness of an aerosol reduction device for ultrasonic scalers. J Periodontol. 1997;68:45–49.
3. Samaranayake LP. Essentials of Microbiology For Dentistry 2nd ed. Edinburgh: Churchill Livingstone; 1998:263–320.
4. Gross KB, Overman PR, Cobb C, Brockman S. Aerosol generation by two ultrasonic scalers and one sonic scaler: a comparative study. J Dent Hyg. 1992;66:314– 318.
5. Harrel SK,Molinari J : Aerosols and splatter in Dentistry : a brief review of the literature and infection control implications. J Am Dent Assoc 2004;135:429.
6. Silness J, Lo¨e H. Periodontal disease in pregnancy.II. Correlation between oral hygiene and periodontalcondition. Acta Odontol Scand 1963;21:533-551.
7. Bently CD, Burkhart NW, Crawford JJ. Evaluating splatter and aerosol contamination during dental procedures. JADA 1994; 125:579-584..
8. Miller RL. Generation of airborne infection by high speed dental equipment. J Am Soc Prev Dent 1976;6:14-17.
9. Micik RE, Miller RL, Mazzarella MA, Ryge G. Studies of aerobiology: bacterial aerosols generated during dental procedures. J Dent Res 1968; 48: 49-56.
10. Pippen DJ, Verderame RA, Weber KK. Efficacy of face masks in preventing inhalation of airborne contaminants. J Oral Maxillofac Surg. 1987; 45: 319-323.
11. Cottone JA, Terezhalmy GT, Molinari JA. Practical infection control in dentistry. Baltimore: Williams and Wilkins; 1996; 2:139-140.
12. Miller RL. Characteristics of blood containing aerosols generated by common powered dental instruments. Am Ind Hyg Assoc J 1995; 56:670-676.
13. Gruninger SE, Siew C, Chang SB. Infection among dentists. JADA1992;123:57-64.
14. Barnes JB, Harrel SK, Hidalgo Rivera F. Blood contamination of the aerosols produced by in vivo use of ultrasonic scaler. J Periodontol 1998; 69:434-438.
15. Earnest R, Loesche W. Measuring harmful levels of bacteria in dental aerosols. JADA 1991; 122:55-57.
16. Holbrook WP, Muri KF, Macphee IT, Ross PW. Bacteriological investigation of the aerosols from ultrasonic scalers. Br Dent J 1978; 144:245-247.
17. Logothetis DD, Martinez-Welles JM. Reducing bacterial aerosol contamination with chlorhexidine gluconate pre-rinse. JADA 1995, 126:1634-1639
18. Briner W.W, Grossman E, Buckner R.Y, Rebitski G.F, Sox T.E, Setser R.E, Ebert M.L. Effect of chlorhexidine gluconate mouth rinse on plaque bacteria. J Perio Res 1986;21:44-52.
19. Nagarale G, Nagarale RG. Chlorhexidine gluconate pre-procedural rinse and bacterial aerosol contamination during ultrasonic scaling. IJPAS 2015;2(2):19-30
20. Johnston JR, Butchart AM, Kgampphe SJ. A comparison of sampling methods for airborne bacteria. Environ Res 1978; 16: 279-284.
21. Fine DH, Yip J, Furgang D, Barnett ML, Olshan AM & Vincent J. Reducing bacteria in dental aerosol: pre-procedural use of an antiseptic mouthrinse. JADA1993; 124:56-58.
22. Klyn SL, Cummings DE, Richardson BW, Davis RD. Reduction of bacteria containing spray produced during ultrasonic scaling. Gen Dent 2001; 49:648-652.
23. Pratten J, Barnett P, Wilson M. Composition and susceptibility to chlorhexidine of multispecies
biofilms of oral bacteria. Appl Environ Microbiol 1998; 64:3515-3519.
24. Gilbert P, Allison DG. Biofilms and their resistance towards antimicrobial agents. In Newman HN, Wilson M, Editors: Dental plaque revisited, New York, 1999, Cambridge University Press, page 125.
25. Larato DC, Ruskin PF, Martin A, et al. Effect of a dental air turbine drill on the bacterial counts in air. J Prosthet Dent 1966;16:758-765.
26. Weeks C, Briner W, Rebitski G, Vick V, Feller M. Immediate and prolonged effect of 0.12% chlorhexidine on salivary bacteria. J Dent Res 1988; 67:326-329.
27. Stephen KH. Contaminated dental aerosol. Dimensions of Dental Hygiene 2003;1(6):16, 18, 20.
28. Stephen KH, John M. Aerosols and splatter in dentistry: A brief review of the literature and infection control implications. JADA 2004; 135.

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Published

2018-01-24

How to Cite

Arora, S. A., Chhina, S., Sahay, D., Puri, G., Kaur, N., & Bhardwaj, D. (2018). Evaluation Of Efficacy Of 0.2% Chlorohexidine Mouthwash As A Pre-Procedural Rinse In Reduction Of Streptococcal Colonies Following Ultrasonic Scaling: A Pilot Study: Evaluation Of Efficacy Of 0.2% Chlorohexidine Mouthwash As A Pre-Procedural Rinse In Reduction Of Streptococcal Colonies Following Ultrasonic Scaling: A Pilot Study. National Journal of Integrated Research in Medicine, 7(5), 46–52. Retrieved from http://nicpd.ac.in/ojs-/index.php/njirm/article/view/1139

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