Evaluation And Comparison Of Antimicrobial Activity Of Tulsi (Ocimum Sanctum), Neem (Azadirachta Indica) And Triphala Extract Against Streptococcus Mutans&Lactobacillus Acidophilus: An In Vitro Study
Activity Of Tulsi, Neem & Triphla
DOI:
https://doi.org/10.70284/njirm.v5i4.759Keywords:
Lactobacillus Acidophilus, Neem, Streptococcus Mutans, Triphala, TulsiAbstract
Background and Objectives: This in vitro study was designed to evaluate and compare the antimicrobial effects of extracts of Neem sticks, Tulsi leaves and Triphala against Streptococcus Mutans and Lactobacillus Acidophilus.Materials and Methods: Sterile solutions of 10% & 25% concentrations were prepared from Neem, Tulsi and Triphala extracts. After growing on culture plates micro-organisms were transferred to nutrient agar; antimicrobial activity of the extracts was tested after 48 h. Results: Mean zones of inhibition against Streptococcus Mutans&Lactobacillus Acidophilus after 24hrs for 10% neem extract is 0.7 & 0.2mm and for 25% neem extract is 1.1 & 0.9 mm respectively. For 10% tulsi extract is 0.3 & 0.2mm and for 25% tulsi extract is 0.5 & 0.6 mm respectively. And for 10% triphala extract is 0.0 & 0.0mm and for 25% triphala extract is 0.1 & 0.0 mm respectively. Interpretation and conclusion: Extracts of neem and tulsi demonstrated an antimicrobial activity but triphla has been failed to show antimicrobial activity against S Mutans and L Acidophilus. This in vitro evaluation is an attempt to encourage further studies comparing the antimicrobial effects of different ayurvedic extracts on prevalence of caries. [Shah SNJIRM 2014; 5(4) :17-21]
References
2. Srinagesh J, Krishnappa P, Somanna SN. Antibacterial efficacy of triphala against oral streptococci: An in vivo study. Indian Journal of Dental Research. 2012; 23(5).
3. Prashant GM, Chandu GN, Murulikrishna KS, Shafiulla MD. The effect of mango and neem extract on four organisms causing dental caries: Streptococcus mutans, Streptococcus salivavius, Streptococcus mitis, and Streptococcus sanguis: An in vitro study. Indian J Dent Res. 2007; 18(4).
4. Subramaniam P, Eswara U, Reddy MKR. Effect of different types of tea on Streptococcus mutans: An in vitro study. Indian Journal of Dental Research; 2012: 23(1).
5. Fitzgerald RJ, Keyes PH. Demonstration of the etiologic role of streptococci in experimental caries in the hamster. J Am Dent Assoc, 1960; 61: 9–18.
6. Shaw JH. Causes and Control of dental caries. N Engl J Med, 1987; 317(16): 996–1004.
7. Ananthanarayan R, Panikar CKJ. Textbook of microbiology. 7th edition. India: Orient Longman; 2005.
8. Coykendall AL. Classifi cation and identifi cation of the Viridans streptococci. Clinical Microbiology Reviews, 1989; 2(3): 315–28.
9. Islam B, Khan SN, Khan AU. Dental caries: From infection to prevention. Med Sci Monit, 2007; 13(11).
10. Elangovan A, Muranga J, Joseph E. Comparative evaluation of the antimicrobial efficacy of four chewing sticks commonly used in South India: An in vitro study. Indian Journal of Dental Research. 2012; 23(6).
11. Agarwal P, Nagesh L, Murlikrishnan. Evaluation of the antimicrobial activity of various concentrations of Tulsi (Ocimum sanctum) extract against Streptococcus mutans: An in vitro study. Indian J Dent Res. 2010 ; 21(3).
12. Dalirsani Z, Aghazadeh M, Adibpour M, Amirchaghmaghi, Paketrat A, Mosannen P et al. Journal of applied sciences. 2011 ; 11(5) : 878-882.
13. Aswini Y Balappanavar, Varun Sardana1, Malkeet Singh. Comparison of the effectiveness of 0.5% tea, 2% neem and 0.2% chlorhexidine mouthwashes on oral health: A randomized control trial. Indian Journal of Dental Research. 2013; 24(1).
