Blood Grouping With ABD PAD- Efficacy And Comparison With Standard Method – A Pilot Study

Blood Grouping With ABD PAD

Abstract

Background: In transfusion service, it is very important to carry out blood group with perfection. Blood grouping is a fast and easy way to ensure that you receive the right kind of blood for right patient. There are many methods we use for blood grouping like Slide method, test tube method, column agglutination method and erythrocyte magnetic technique. Test tube method is gold standard method for blood grouping. We need to verify donor blood group in blood bank or at camp site, for patients at bed side, at the time of issue of blood unit or at the time of dire emergency so to verify the blood group in Blood centres we require the results of blood grouping urgently. ABD PAD® is a new device for the quick confirmation of the ABO blood group. Materials And Methods: In this Pilot study,200 samples were taken of which 100 were donor samples and 100 were patient samples. Blood grouping was performed on the ABD PAD, and results were compared with conventional tube technique and automate platform, Qwalys 3 (Diagast) EMT technique.Results: All 200 tests performed on the ABD PAD were concordant with other standard methods, namely the Test Tube technique and automated platform, Qwalys 3 (Diagast).The results of ABD PAD were quicker(within 30 seconds) and easy to interpret.Discussion:The manual confirmation of the ABO group and RH- rhesus is a repetitive and time consuming task. It requires following a procedure handling several reagents. In this context, the ABD pad is a ready to use device using the latex technology M- TRAP, facilitating the procedure intended for the confirmation of ABO/Rh. The only drawback is that we are not able to perform reverse grouping. Conclusion: ABD PAD is very useful for blood grouping in donors in the blood bank as well as in outdoor blood donation camp and at the time of issue of blood unit when we want to re-check the blood group of the issue bag. It is easy to interpret, fast, reliable and results are comparable with standard methods. [Shah M Natl J Integr Res Med, 2021; 12(1): 42-45]