Study OfAgNOR Count In FNAC of Breast Lesions
Study Of AgNOR Count In FNAC of Breast Lesions
Keywords:
AgNOR, Fine needle aspirate, SAPA scoreAbstract
Background:ArgyrophilicNucleolar Organizer Region technique has a potential value in the diagnosis of malignancy and can be used in cases with equivocal and inconclusive cytological picture. The purpose of this study was to evaluate mean ArgyrophilicNucleolar Organizer Region count and ArgyrophilicNucleolar Organizer Region Pattern Assessment score in fine needle aspirates of breast lumps.Materials and Methods: The present study consists of 80 cases of AgNOR count done in fine needle aspiration cytology of various breast lesions in patients on O.P.D. basis or those admitted in Guru Govindsingh Hospital attached to Shri M.P. Shah Medical College, Jamnagar during the period between August '98 to May 2000. Fine Needle Aspiration smears were studied by conventional methods and silver staining for ArgyrophilicNucleolar Organizer Regions. Histopathologic diagnosis was taken as the gold standard. Results:ArgyrophilicNucleolar Organizer Region count and ArgyrophilicNucleolar Organizer Region Pattern Assessment score were helpful in differentiating benign from malignant lesions. Mean ArgyrophilicNucleolar Organizer Region count and ArgyrophilicNucleolar Organizer Region Pattern Assessment score were 2.63 ± 1.36 and6.26 ± 1.19 respectively in benign lesions while they were 8.42 ± 2.53 and 10.05 ± 2.22 respectively in malignant lesions.With few exceptions, cases with high counts had high scores. Conclusion: Mean ArgyrophilicNucleolar Organizer Region AgNOR count and Subjective ArgyrophilicNucleolar Organizer Region Pattern Assessment score provide useful information regarding cellular proliferation. Both count and score have comparable diagnostic potential but the latter is a more convenient and rapid method for ArgyrophilicNucleolar Organizer Region evaluation.
References
2. Crocker J, Egan MJ. Correlation between NOR sizes and numbers in non-Hodgkins lymphomas. JPathol. 1988;156:233-9.
3. Contractor H, Ruschoff J, Hanisch T, Ulshofer B,
Neumann K, Schultze-Seemann W, Thomas C.Silver stained structures in prostatic carcinoma:evaluation of diagnostic and prognostic relevanceby automated image analysis. Urol Int. 1991;46:9-14.
4. Khanna AK, Datta G, Kumar M. Correlation ofAgNOR with tumor size, stage of tumor, lymphnodestatus and grade of tumor in oral cancer. In: VermaAK, editor. Oral Oncology IV B. Bangalore, India:MacMillan India, 1995:25-30.
5. Khanna AK, Giri AK, Khanna A, Kumar M. Nucleolarorganizer region count and subjective AgNORpattern assessment (SAPA) score in skin tumors. JSurgOncol. 2001;78:273-8.
6. Giri DD, Nottingham JF, Lawry J, Dundas SAC, Underwood JCE. Silver-binding nucleolar organizerregions (AgNORs) in benign and malignant breastlesions: correlation with ploidy and growth phaseby DNA flowcytometry. J Pathol. l989;157:307-13.
7. Egan MJ, Crocker J. Nucleolar organizer regions inpathology. Br J Cancer. 1992;65:1-7.
8. Kumar Anand, Kushwaha Kumar Anand, Kumar Mohan, Gupta Saroj : ArgyrophilicNucleolar Organizer Regions. Their value and correlation with clinical prognostic factors in breast carcinoma. Journal of Surgical Oncology 1997; 65 : P 201-204.
9. Sivridis E. and Sims B. :Nucleolar Organizer regions new prognostic variables in breast carcinomas. J. Clin. Pathol. 1990 ; 43 : P 390-392.
10. Bankfalvi A., Ofner D., Schmid K. W., Schmitz K. J., Breukelmann D., Krech R., Bocker W. : Standardized in Situ AgNOR Analysis in Breast Pathology – diagnostic and Cell Kinetic Implications Pathology Res. Pract. 1999; 195 : P 219 – 229.
11. Rajeevan K/ Aravindan KP, and Chandrika K. : Value of AgNORs in fine needle aspiration cytology of breast lesions J. Pathol. Microbiol1995 ; 38 : P 17-24.
12. Dervan P. A., Gilmartin L. g., Loffus B. M. and Carnery D.N. : AgrytophilicNucleolar OrganizerRegion counts correlate with Ki-67 scores. Am. J. Clin, Pathol. 1989 ; 92 : P 401 – 407.
13. Karmaker T., Radhika S., Gupta S. K. :Argyrophilicnucleolar organizer regioins( AgNORs ) in breast lesions - a study on fine needle aspirates. Cytopathology 1995; 6 : P 5-13.
14. Dasgupta Anjali, Ghosh R.N., SarkarRanu, Laha R. N., Ghosh T. K., Mukherjee Chhanda : ArgyrophilicNucleolar Organizer Regions ( AgNORs) in Breast Lesions. Journal of Indian Medical Association 1997 ; 95 : P 492 -494.
15. Agarwal P. K., MehrotraAnju, ChardraTulika : Diagnostic relevance of silver stained nucleolar organizer region ( AgNORs) in benign and malignant breast lesions. Indian Journal of Experimental Biology 1995; 33: p 715-720.
