Salivary Total Antioxidant Activity as a Non Invasive Biomarker for Oxidative Stress In Asthmatic Patients
Salivary Total Antioxidant Activity as a Non Invasive Biomarker for Oxidative Stress
DOI:
https://doi.org/10.70284/njirm.v3i1.1957Keywords:
Bronchial asthma, oxidative stress, saliva, total antioxidant activityAbstract
Background: Bronchial Asthma is a chronic inflammatory disease along with hyperresponsiveness of bronchi. The present study was designed to assess the redox status of patients suffering from bronchial asthma and to compare it with that of normal healthy controls, by determining the total antioxidant activity (AOA) of the serum and saliva and correlating it with the disease status. Method: Total AOA was assayed spectrophotometrically in saliva and serum of two groups; asthmatic patients attending OPD of pulmonary Medicine and healthy controls. The patients were followed for a period of three months after start of therapy and total AOA was measured post therapy. Results: Asthmatic patients exhibited significantly(p<0.05) decreased serum and salivary total AOA as compared to healthy controls. Decreased contents of total AOA in serum and saliva was positively correlated with the severity of disease process. Total AOA in serum was significantly (p<0.0001) higher than salivary AOA in all the categories of asthmatic patients. Total AOA in serum was significantly (p<0.0001) higher than that in saliva of the control subjects. The depressed total AOA returned to near normal values post treatment. In Conclusion: Total AOA in serum and saliva is a good indicator for assessing the severity and progress of bronchial asthma. Salivary total AOA can be taken as a potential biomarker for oxidative stress in asthmatic patients.
References
2. Caramory G, Papi A. Oxidants and Asthma. Thorax 2004; 59; 1703.
3. Humbert M, Ying S, Corrigan CJ, Robinson DS. The immunopathology of extrinsic (atopic) and intrinsic (non-atopic) asthma. Immunol Today 1999; 20: 528- 33.
4. Ahmed N, Hanumanthrao G, Chhabra S. Increased oxidative stress in acute exacerbations of asthma. Journal of Asthma 2005; 1: 45-50.
5. Hisatoshi S, Masakazu I . Oxidative & nitrative stress in bronchial asthma. Antioxidants & redox signaling 2008; 10 (4); 785- 798
6. Zhao JJ, Shimizu Y, Dobashi K, Kawata T, Ono A, Yanagitani N. The relationship between oxidative stress and acid stress in adult patients with mild asthma. J Investing Allergol Clin Immunol 2008; 18(1): 41-45.
7. Fujisawa, T. Role of oxygen radicals on bronchial asthma. Current drug targets- Inflammation & allergy 2005; 4 : 505–509
8. Nadeem A, Chhabra SK, Masood A, Raj HG. Increased Oxidative stress and altered levels of antioxidants in asthma. J allergy Clin Immunol 2003; 111(1): 72-78.
9. Carlos K and Bucalen F. Total antioxidant capacity: a biomarker in biomedical and nutritional studies. Journal of cell and molecular biology 2008; 7(1): 1-15.
10. Radhakrishnan S, Narayanasamy A, Jyotirmay B, Sivaramakrishnan R. Total antioxidant capacity in Eale’s disease, uveitis & cataract. Indian J Med Res 134, July 2011; 83-90.
11. Ruchi K, Anil Kumar S, Sunil G, Bashir A, Prabhat S. Antioxidant activity in children with ADHD –a comparison in Untreated and treated subjects with normal children. The International Medical Journal Malaysia, june 2011; 10,1.
12. Meucci E, Littarru C, Deli G, Luciani G, Tazza L, Littarru GP. Antioxidant status and dialysis; plasma and saliva antioxidant activity in patients with fluctuating urate levels. Free Radic Res 1998; 29(5): 367-76.
13. Moore S, Calder KA, Miller NJ. Rice–Evans CA. Antioxidant activity of saliva and periodontal disease. Free Radic Res 1994; 21 (6): 417-25.
14. David WR, David PJ, Bryce F, Chris W, Hayden W. Exhaled nitric oxide continues to reflect airway hyperresponsiveness and disease activity in inhaled corticosteroid- treated adult asthmatic patients. Respirology 2003; 8: 479.
15. Fabio L, Ricciardolo M, Peter J, Sterk, Benjamin G, Folkerts G, Nitric oxide in Health and Disease of the Respiratory Systems. Physiol. Rev 2004; 84: 731-765.
16. Wood LG, Gibson PG, Garg ML. Biomarkers of lipid peroxidation, airway inflammation and asthma. Eur Respir J 2003; 21: 177-186.
17. Mani SK, David ML, Serpil CE. Asthma. Department of Pulmonary Allergy and Critical Care Medicine 2005. The Cleveland Clinic.
18. Picardo C, Deulofeu R, Lleonart R, Agusti M, Mullol J, Quinto l, et al. Dietary micronutrients/ antioxidants and their relationship and bronchial asthma severity. Allergy 2001; 56(1): 43-49.
19. Bentur L, Mansour Y, Brik R, Eizenberg Y, Nagler R. Salivary oxidative stress in children during acute asthmatic attack & during remission. Respiratory medicine 2006; 100(7): 1195- 1201.
20. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Methods for the measurements of antioxidant activity in human fluids; J Clin Pathol 2001; 54: 356-61.
