Assessment Of Cytotoxicity Of Chlorhexidine Containing Mouthrinses By Micronucleus Test In Exfoliated Buccal Epithelial Cells

Cytotoxicity Of Chlorhexidine By Micronucleus Test


  • Dr. Vinodkumar M P
  • Dr. Shrinivas S. Vanaki
  • Dr. Rudrayya S. Puranik


micro nucleus test, chlorhexidine, exfoliative cytology, cytotoxicity, Feulgen reaction


Background & Objective: Chlorhexidine mouth rinses are utilized worldwide on regular basis in oral
hygiene practice for plaque control. Studies have shown that chlorhexidine has toxic effects on a variety of eukaryotic
cells. Micronuclei count in exfoliative cells is an economical and non invasive diagnostic method for evaluation of
cytotoxic effects of many carcinogens/ co-carcinogens. The present study was conducted in order to evaluate
frequency of micronuclei in buccal epithelial cells of patients using chlorhexidine containing mouthrinses.
Methodology: Study included 50 subjects, of whom 30 having clinically suspected mild gingivitis, rinsed with
chlorhexidine; 20 control subjects, rinsed with saline. Buccal epithelial cells were collected with a brush before and
after one week of usage of chlorhexidine by patients and physiologic saline by controls. Cells subjected to Fuelgen
reaction and analysed by two independent observers for micronuclei counts. Results: Considerable increase in
micronuclei count was noted in patients using chlorhexidine compared to controls. Conclusion: Present study
suggests that chlorhexidine has cytotoxic effects and further studies involving the therapeutic use of different
mouthrinses for a longer duration may provide justification for their usage in clinical practice. [Vinod Kumar MP
NJIRM 2016; 7(3): 75 - 79]


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How to Cite

M P, D. V., Vanaki, D. S. S., & Puranik, D. R. S. (2018). Assessment Of Cytotoxicity Of Chlorhexidine Containing Mouthrinses By Micronucleus Test In Exfoliated Buccal Epithelial Cells: Cytotoxicity Of Chlorhexidine By Micronucleus Test. National Journal of Integrated Research in Medicine, 7(3), 75–79. Retrieved from



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