Prevalence Of Human Brucellosis Among High Risk, Symptomatic Cases In Anand District Of Gujarat-India

A Study Of Human Brucellosis In Anand District

Authors

  • Dr Suman Singh
  • Dr Shah Krutharth K
  • Ms Kothari Shikha Y

Keywords:

human brucellosis, high risk individuals, prevalence

Abstract

Because of nonspecific and chronic symptoms, diagnosis requires high clinical suspicion and laboratory confirmation. Prevalence of brucellosis in India, more so in Anand district, is not well studied. This study was conducted as a pilot to find prevalence of human brucellosis among high risk symptomatic cases along with the clinical profile and associated risk factors. Methodology: This is a cross-sectional descriptive study conducted in Anand district of Gujarat, India. Samples from 50 high risk symptomatic cases were subjected to serology, blood culture and genome detection by conventional Polymerase Chain Reaction (PCR) using B4/B5 primers, for detection of brucella infection. Data was analyzed using Epi Info software (version: 3.5.1). Results: We detected sero-prevalence of 14% and genome detection of 16% with no isolation in blood culture. Among them four (8%) were positive by serology and PCR both. Titers in seropositive cases ranged from 1:40 to 1:160. Fever was the most common clinical complain among sero-positive (71.4%) and PCR positive (62.5%) cases. History of direct contact with cattle was present in all serology and PCR positive patients. Consumption of unpasteurized milk or milk products (86%), consumption of uncooked or partially cooked meat (28.5%) and working as veterinarian (14.3%) were other risk factors identified. Conclusions: Thus we found brucellosis to be a significant health problem. A larger Indian study with follow up of positive cases, comparative study for standardization of various diagnostic modalities and response to therapy is strongly needed. [Singh S NJIRM 2015; 6(5):22-27]

References

1. Mantur BG, Biradar MS, Bidri RC, Mulimani MS, Veerappa, Kariholu P, et al. Protean clinical manifestations and diagnostic challenges of human brucellosis in adults: 16 years experience in an endemic area. J Med Microbiol. 2006;55:897-903
2. Dean, Anna S., Lisa Crump, Helena Greter, Jan Hattendorf, Esther Schelling, and Jakob Zinsstag. "Clinical manifestations of human brucellosis: a systematic review and meta-analysis." PLoS Negl Trop Dis (2012): 6(12): 1929
3. Mantur BG, Amarnath SK, Shinde R. Review of clinical and laboratory features of human brucellosis. Indian J Med Microbiol. 2007;25 (3):188-202.
4. Mantur BG, Amarnath SK. Brucellosis in India-a review. J Biosci. 2008;33: 539-47.
5. Pappas G, Papadimitriou P, Akritidis N, Chrisou L, Tsianos EV. The new global map of human brucellosis. Lancet Infect Dis. 2006;6: 91-99.
6. Dean AS, Crump L, Greter H, Schelling E, Zinsstag J (2012) Global Burden of Human Brucellosis: A Systematic Review of Disease Frequency. PLoS Negl Trop Dis 6(10): e1865. doi:10.1371/journal.pntd.0001865
7. Seven neglected endemic zoonoses - some basic facts, available at http://www.who.int/zoonoses/neglected_zoonotic_diseases/en/
8. Corbel MJ. Brucellosis: an overview. Emerg Infect Dis. 1997; 3: 213-21.
9. Singh SP, Parikh SS, Sero-prevalence of human brucellosis in high risk population of Anand District. International Journal of Research in Medicine. 2014:3(1);36-38
10. Leal-Klevezas DS, Martinez-Vazquez OI, Lo´ pez-Merino A. and Martinez-Soriano JP Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J Clin Microbiol 1995;33: 3087–90.
11. Baddour MM, Alkhalifa DH. Evaluation of three polymerase chain reaction techniques for detection of Brucella DNA in peripheral human blood. Can J Microbiol. 2008:54(5);352-7.
12. Sharma VK, Savalia CV. Seroprevalence of brucellosis in human population in Gujarat. Indian Journal of Field Veterinarians. 2009: 4(3);5-6.
13. Panjarathinam R, Jhala CI. Brucellosis in Gujarat state. Indian J Pathol Microbiol. 1986;29(1):53-60.
14. Appannanavar SB, Sharma KB, Verma S, Sharma M. Seroprevalence of brucellosis: A 10-year experience at a tertiary care center in north India. Indian J Pathol Microbiol. 2012;55(2):271-72.
15. Handa R, Singh S, Singh N, Wali JP. Brucellosis in north India: Results of a prospective study. J Commun Dis. 1998;30:85-87.
16. Sen MR, Shukla BN, Goyal RK. Seroprevalence of brucellosis in and around Varanasi. J Commun Dis. 2002;34:226-27.
17. Kadri SM, Rukhsana A, Laharwal MA, Tanvir M. Seroprevalence of brucellosis in Kashmir (India) among patients with pyrexia of unknown origin. J Indian Med Assoc. 2000;98:170-71.18. Parizadeh SM, Seyednozadi M, Erfanian MR, Nezhad MA. A Survey on Antibody Levels among Individuals at Risk of Brucellosis in Khorasan Razavi Province, Iran. Pak J Nutri. 2009;8(2):139-44.
19. Young EJ. ‘Serologic diagnosis of human brucellosis: Analysis of 214 cases by agglutination tests and review of the literature’. Rev Infect Dis 1991;13:359-72.
20. Espinosa BJ, Chacaltana J, Mulder M, Franco MP, Blazes DL, Gilman RH, et al. Comparison of Culture Techniques at Different Stages of Brucellosis. Am. J. Trop. Med. Hyg. 2009;80(4):625–27.
21. Alsayed Y, Monem F. Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations? J Infect Dev Ctries. 2012;6(6):495-500.
22. Elfaki MG, Uz-Zaman T, Al-Hokail AA, Nakeeb SM. Detection of Brucella DNA in sera from patients with brucellosis by polymerase chain reaction. Diagn Microbiol Infect Dis. 2005;53(1):1-7.
23. Castano MJ, Solera J. Chronic Brucellosis and Persistence of Brucella melitensis DNA. J Clin Microbiol. 2009;47(7):2084-89.
24. Mukhtar F. Brucellosis in a high risk occupational group: seroprevalence and analysis of risk factors. J Pak Med Assoc.2010;60(12): 1031-34.

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Published

2018-01-11

How to Cite

Singh, D. S., K, D. S. K., & Y, M. K. S. (2018). Prevalence Of Human Brucellosis Among High Risk, Symptomatic Cases In Anand District Of Gujarat-India: A Study Of Human Brucellosis In Anand District. National Journal of Integrated Research in Medicine, 6(5), 22–27. Retrieved from http://nicpd.ac.in/ojs-/index.php/njirm/article/view/960

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