Estimation Of Plasma Levels Of Lipid Peroxidation And Erythrocyte Enzymatic Antioxidants In Type II Diabetes Without Complications
Lipid Peroxidation And Erythrocyte Enzymatic Antioxidants In Type II Diabetes Without Complications
DOI:
https://doi.org/10.70284/njirm.v6i5.959Keywords:
diabetes, oxidative stress, antioxidants, MDAAbstract
Background & Objective: Diabetes being a chronic disorder is associated with increased oxidative stress by glucose oxidation and non enzymatic protein glycation. Imbalance between oxidants and antioxidants lead to oxidative stress. Increased levels of malondialdehyde (MDA) end product of lipid peroxidation have been observed in type II diabetes but results regarding status of enzymatic antioxidants, Glutathione peroxidase (GPx) and Glutathione reductase (GR) are conflicting. Therefore this study was conducted to estimate levels of MDA, GPX & GR and to find the correlation of these to glycaemic control if any in diabetics without complications. Methodology: Type II diabetics (n=40) diagnosed not more than 5 yrs back, without any complications were cases and age & sex matched normal subjects (n=40 ) were controls in this study. Blood levels of glucose, glycosylated Hemoglobin (HbA1C), MDA and erythrocytic activity of GPX and GR were estimated. Results: Blood MDA level was found to be significantly increased and erythrocyte GPx & GR activity was decreased significantly. A strong positive correlation was seen between MDA and HbA1C. Conclusion: Thus imbalance between MDA and antioxidant activity indicate that the increased oxidative stress is present even in early period of diabetes without any complications and glycaemic control may help in reducing oxidative stress and further complications. [Ghugare B NJIRM 2015; 6(5):17-21]
References
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27. Madhur Gupta and Suresh Chari. Prooxidants and antioxidants in patients of type II diabetes mellitus with Ischaemic heart disease. Indian J. Of Clin. Biochemistry 2006;21(1) :118-122.
28. Murakami K, Konto T, Ohtsuka Y, Fujiwara Y, Shimada M, Kawakami Y. Impairment of glutathione metabolism in erythrocytes from the patients with diabetes mellitus. Metabolism 1989; 38(8):753-8.
29. Abou-Seif MA, Youssef AA. Oxidative stress and male IGF-1, gonadotropin and related hormones in diabetic patients. Clin Chem Lab Med. 2001;39(7):618-23.
30. Komosioska-Vassev K, Olczyk K, Olczyk P, Winsz-Szczotka K. Effects of metabolic control and vascular complications on indices of oxidative stress in type 2 diabetic patients. Diabetes RES Clin Pract. 2005;68(3):207-16.
2. John W. Baynes et al. Role of oxidative stress in diabetic complications. Diabetes1999; 25(2):1-9.
3. Giugliano D, Ceriello A. Oxidative stress and diabetic vascular complications. Diabetes Care 1996; 30(1):257-267.
4. Dichey,F.H, Cheland G.H, and Lotz C.(1949).The role of organic peroxides in the induction of mutations. Proceedings of Natural Academy of Sciences of United State of America 1949; 35:581.
5. K. H. Cheesman, T.F, Slater. An introduction to free radical biochemistry.British Medical Bulletin 1993;49(3);481-493.
6. Alti T, Keven K, Avei A, Kutlav S, Turkeapar N, Varli M, Aras S. Ertug E, Cambolat O. Oxidative stress and antioxidant status in elderly diabetes mellitus and glucose intolerance patients. Arch Gerontol Geriatr 2004;39(3):269-75.
7. Turk TM, Sevinc A, Camci C, Cigli A. Buykbeber S, Savli H, Bayraktar N. Plamsa lipid peroxidation products and antioxidant enzymes acivity in patients of type 2 diabetes mellitus. Acta Diabetol 2002;39(3):117-22.
8. Nakhjavani M,,Esteghamti A, Nowroozi, et al .Type 2 diabetes mellitus duration : an independent predictor of serum malondialdehyde levels.Singapure med J 2010;51(7):582
9. S.Merzouk, A. Hichami, S. Madani, H. Merzouk, A. Y. Berrouiguet, J. Prost, K. Moutairou, N. ChabanSari and N.A.Khan. Antioxidant status and levels of different vitamins determined by high performance liquid chromatography in diabetic subject with multiple complications.Gen. Physiol Biophys 2003;22:15-27.
10. Dave GS, Kalia K. Hyperglycemia induced oxidative stress in type 1 and type 2 diabetic patients with and without nephropathy. Cell Mol Biol 2007; 53(5):68-78.
11. M.Rema,V Mohan, Anusha Bhaskar, and K Radha Shanmugasundaram.Does oxidative Stress Play a Role in Diabetic Retinopathy? Indian J Opthalmol1995; 43:17-21.
12. Kaji H, Kurasaki M, Ito K, Saitro J, Saito K, Niiko T, Kojima Y, Ohsaki Y, Ide H, Tsuji M et al. Increased lipoperoxide value and glutathione peroxidase activities in blood plasma of type 2 diabetic women. Clin Wochenschr 1985;63(16):765-8.
13. Taheri E, Djalali M, Saedisomeolia A, et al. The relationship between theactivates of antioxidant enzymes in red blood cells and body mass index in Iranian type 2 diabetes and healthy subjects. Journal of Diabetes & Metabolic Disorders 2012, 11:3 http://www.jdmdonline.com/content/11/3
14. Trinder P. Glucose oxidase method. Arch Clin Biochem 1969; 6:24.
15. K.Michael Parker,Jack D England,Jose De Costa, Randall L Hess and David E Goldestein.Improved colorimetric assay for Glycosylated hemoglobin.Clin Chem 27(5),669-670,1981.
16. Satoh K: Serum lipid peroxide in cerebrovascular disorders determined by new colorimetric method. Clin Chem Acta 1978;90:37-43
17. Jakus V. The role of free radicals in oxidative stress and antioxidants systems in diabetic vascular disease, Bratist Lek Listy 2000; 101(10):541-551.
18. Arindam Saha, Sangeeta Adak, Subhamkar Chowdhury and Maitree Bhattacharyya. Enhanced oxygen releasing capacity and oxidative stress in diabetes mellitus. Clinica Chimica Acta 2005;361(1-2):141-149.
\19. Robert Murray et al (eds.) Harper’s biochemistry.25th edition, Philadelphia 2014; p.no.72.
20. Dichey FH, Cheland GH, and Lotz C. The role of organic peroxides in the induction of mutations. Proceedings of Natural Academy of Sciences of United State of America 1949;35:581.
21. Song F, Jia W, Yao Y, Hu Y, Lei L, Lin J. Sun X, Liu L. Oxidative stress, antioxidant status and DNA damage in patients with impaired glucose regulation and newly diagnosed type 2 diabetes. Clin. Sci. (Lond) 2007;112(12):599-606.
22. Philips M, Cataneo R N, Cheema T, Greenberg J. Increased breath biomarkers of oxidative stress in diabetes mellitus. Clinica Chimica Acta 2004; 344:189-194.
23. American Diabetes Association. Standards of medical care in diabetes. Diabetes Care 2014; 37:14–80.
24. Jonas, Nordberg and Elias S.J. Arner. Reactive oxygen species, antioxidants and the mammalian thioredoxin system. Free radical Biology and Medicine 2001;31(11):1287-1312.
25. M.Rema,V Mohan, Anusha Bhaskar, and K Radha Shanmugasundaram.Does oxidative Stress Play a Role in Diabetic Retinopathy? Indian J Opthalmol 1995;43:17-22.
26. Dichey,F.H, Cheland G.H, and Lotz C. The role of organic peroxides in the induction of mutations. Procedings of Natural Academy of Sciences of United State of America 1949; 35:581.
27. Madhur Gupta and Suresh Chari. Prooxidants and antioxidants in patients of type II diabetes mellitus with Ischaemic heart disease. Indian J. Of Clin. Biochemistry 2006;21(1) :118-122.
28. Murakami K, Konto T, Ohtsuka Y, Fujiwara Y, Shimada M, Kawakami Y. Impairment of glutathione metabolism in erythrocytes from the patients with diabetes mellitus. Metabolism 1989; 38(8):753-8.
29. Abou-Seif MA, Youssef AA. Oxidative stress and male IGF-1, gonadotropin and related hormones in diabetic patients. Clin Chem Lab Med. 2001;39(7):618-23.
30. Komosioska-Vassev K, Olczyk K, Olczyk P, Winsz-Szczotka K. Effects of metabolic control and vascular complications on indices of oxidative stress in type 2 diabetic patients. Diabetes RES Clin Pract. 2005;68(3):207-16.
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Published
2018-01-11
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Meshram, D. R. S., Ghate, D. J. R., Ghugare, D. B. W., Koundinya, D. S., & Parmar, D. D. J. (2018). Estimation Of Plasma Levels Of Lipid Peroxidation And Erythrocyte Enzymatic Antioxidants In Type II Diabetes Without Complications: Lipid Peroxidation And Erythrocyte Enzymatic Antioxidants In Type II Diabetes Without Complications. National Journal of Integrated Research in Medicine, 6(5), 17–21. https://doi.org/10.70284/njirm.v6i5.959
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