A Prospective Comparative Study of Semi-Quantitative Slide Widal test& Quantitative Tube Widal test
A Prospective Comparative Study of Semi-Quantitative Slide
Keywords:
Salmonella, Slide Widal test, Tube Widal testAbstract
Objective: The diagnosis of enteric fever currently depends upon the isolation of Salmonella from a patient, most commonly by blood culture. This facility is not available in many areas where the disease is endemic. The Widal agglutination test which demonstrates the presence of somatic (O) and flagellar (H) agglutinins to Salmonella in the patient's serum using O and H antigen suspension is one of the most utilized diagnostic tests for typhoid fever in developing countries. In the recent past, a rapid slide test was developed which is now the most commonly used technique in local laboratories because of its convenience. The semi-quantitative slide test provides an approximation to the tube test titre. The present study was an attempt to compare the results of semi-quantitative slide agglutination with the quantitative tube method to ascertain any significant difference between two methods. Material and Methods: 300 precollected blood samples of OPD patients were collected over a period of 4 months and subjected to semi-quantitative rapid slide and quantitative tube Widal tests using commercially available Salmonella antigen test kits. Results: From the 300 samples studied, 17(5.7%) serum samples were positive by slide test method while 25(8.7%) seropositive by Tube test method. Present study shows, slight difference in the results obtained by the two methods of Widal test. Conclusion: The Tube Widal test is more sensitive and specific than slide Widal test, especially for rule out prozone phenomena, but slide Widal agglutination test is found to good alternative screening test because less time consuming, easy to perform, cost effective and can be applied in resource poor nations. There is an urgent need for the rational design and evaluation of effective and appropriate diagnostics for typhoid fever. [Jain M NJIRM 2014; 5(6):73-77]
References
2. Pokhrel BM, Karmacharya R, Mishra SK, Koirala J. Distribution of the antibody titre against Salmonella enterica among healthy individuals in Nepal. Ann ClinMicrobiolAntimicrob 2009; 8:1.
3. Brusch JL, Garvey T, Corales R, Schmitt SK. 2006. eMedicine from WebMd -Typhoid Fever available at http//www.emedicine.com.MED/topic2331.htm.
4. Kidgell C, Reichard U, Wain J, Linz B, Torpdahl M, Dougan G et al. Salmonella typhi, the causative agent of typhoid fever, is approximately 50,000 years old.Infection, Genetics and Evolution 2002; 2: 39-45.
5. Crump JA, Stephen P, Luby ED and Mintz. The global burden of typhoid fever. Bull World Health Organ 2004; 82:1-24.
6. World Health Organization. 6th International Conference on Typhoid Fever and other Salmonelloses. 2006. Geneva, WHO.
7. Dr.ZohraBegumComparisionamong the different diagnostic proceduresforeatlyand rapid diagnosis of typhoid fever (2008)
8. Ananthanarayan and Paniker's. Text book of microbiology.8th edition, 2009; 288-299.
Kenneth Todar, Textbook of bacteriology of Wisconsin. 2008-2012
10. Dr Balakrishna. Comparative study of typhidot and Widal test in the diagnosis of typhoid fever.(2010)
11. Wain J, Bay PVB, Viinh H, Duong NM, Deep TS, Walsh AL, etal.Quantitation of bacteria in bone marrow from patients with typhoid fever:Relationship between counts and clinical features. J Clin Microbial 2001;39(4)1571-5.
12. Yaramis A, Yildirim I, Katar S, Ozbek MN, YalcinT, Tas MA, et al. Clinical and laboratory presentation of typhoid fever. International Paediatr 2001;16(4):227-31
13. Retnosari S, Tumbelaka AR, Akib AP, Hadinegoro SRS. Clinical and laboratory features of typhoid fever in childhood, focus on relationship between prior antibiotic administration with blood culture, Widal and typhidot results. PaediatrIndones 2001;41:149-54.
14. Olsen SJ, Pruckler J, Bibb W, Thanh NTM, Trinh TM, Minh NT et al.Evaluation of rapid diagnostic tests for typhoid fever. JCM 2004; 42:1885-89
15. Chiragh S, Rehman SU, Blood and bone marrow culture sensitivity in clinically suspected enteric fever. J Postgraduate Med Institute 2005;19(3):313-6
16. Bhutta ZA and Mansurali N. Rapid serologic diagnosis of paediatric typhoid fever in an endemic area: A prospective comparative evaluation of two dot-enzyme immunoassays and the Widal test. Am J Trop Med Hyg 1999; 61:654-657.
17. Gopalakrishnan V, Sekhar WY, Soo EH, Vinsent RA, Devi S. Typhoid fever in Kuala Lumpur and a comparative evaluation of two commercial diagnostic kits for the detection of antibodies to Salmonella typhi. Singapore Med 2002;43(7):354-8.
18. Sherwal BL, Dhamija RK, Randhawa VS, Jais M, Kaintura A, Kumar M.A comparative study of typhidot and Widal test in patients of typhoid fever. JIND Acad Cord Med;20045(3):224-6
19. TupasiE.Thelma et al. Clinical Application of the widal test. Phil J Microbiolinfect Dis 1991;20(1):23-6
