Cook Your Own Ampholytes For Use In Isoelectric Focusing And Chromatofocusing

Cook Your Own Ampholytes For Use In Isoelectric Focusing And Chromatofocusing

  • Tejas Kalaria Department of Biochemistry, Medical College Baroda, Vadodara, Gujarat 390 001, India
Keywords: ampholytes, isoelectric focusing, chromatofocusing

Abstract

Isoelectric focusing and chromatofocusing are techniques to separate amphoteric compounds based on their isoelectric points. The commonest used compounds to generate a pH gradient in these techniques are ampholytes. The prohibitively expensive cost of carrier ampholytes limits the application of these techniques in less advantaged parts of the world. A simple and inexpensive method is described in this paper to prepare a broad pH range (pH 3 to 11) ampholytes for use in isoelectric focusing or chromatofocusingin any routine laboratory. The method involves crosslinking polyethylene polyamine to create complex cross-linked polyaminesin the first step. Whereas, the second step involves reacting the cross-linked polyamines with an unsaturated carboxylic acid to produce ampholytes. [Kalaria T Natl J Integr Res Med, 2021; 12(3):105-109]

Published
2021-07-01
How to Cite
Tejas Kalaria. (2021). Cook Your Own Ampholytes For Use In Isoelectric Focusing And Chromatofocusing: Cook Your Own Ampholytes For Use In Isoelectric Focusing And Chromatofocusing. National Journal of Integrated Research in Medicine, 12(3), 105-109. Retrieved from http://nicpd.ac.in/ojs-/index.php/njirm/article/view/3086
Section
Short Communication