Evaluation of Antioxidants and Lipid Peroxidation Status in Rheumatoid Arthritis Patients
Assessment of Oxidative Stress in RA Patients
DOI:
https://doi.org/10.70284/njirm.v4i5.2219Keywords:
Antioxidants, Free radicals, Lipid peroxidation,Rheumatoid ArthritisAbstract
Background and Objectives:Rheumatoid Arthritis (RA) is characterized by a chronic hypertrophic synovitis leading to destruction of connective tissues and functional damage of cartilage and bony structure. Reactive oxygen species play an important role in tissue injury of this disease. The objective of this study was to measure the oxidative stress, enzymatic and non-enzymatic antioxidants in both study and control groups. Materials and methods: The study was conducted on 50 RA patients (35 Female /15 Male) with mean age of (46±6.3) years. Both the study groups were non-smokers and non-alcoholics and were not suffering from any other chronic disease. Lipid peroxidation products, enzymatic and non-enzymatic antioxidants were estimated in cases (50) and controls (30). Results: TBARs levels in patients with Rheumatoid arthritis were found to be significantly (p<0.001) higher than controls, whereas levels of GSH-Px, SOD and CAT, Vitamin E (p<0.001) and vitamin C (p<0.004) were significantly lower in the RA patients compared to control subjects. Uric acid and ceruloplasmin levels in the plasma of RA patients were significantly (p <0.001) higher as compared to control subjects. Conclusion: The enhanced lipid peroxidation accompanied by perturbation in antioxidant status indicates free radical mediated oxidative damage in rheumatoid arthritis.
References
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2. Valko M, Leibfritz D, Moncola J, Cronin MD, et al. Free radicals and antioxidants in normal physiological functions and human disease. Review. Int J BiochemCell.Biol 2007;39:44-84.
3. Walston J, Xue Q, Semba RD, Ferrucci L, et al. Serum antioxidants, inflammation, and total mortality in older women. Am J Epidemiol. 2006;163:18-26.
4. Mahajan A, Tandon VR. Antioxidants and rheumatoid arthritis. J Indian Rheumatol Ass. 2004;12:139-42.
5. Halliwell B, Gutteridge JMC. Free radicals in biology and medicine.4th ed. Oxford, UK: Clarendon Press; 2007.
6. Bahorun T, Soobrattee MA, Luximon-Ramma V, Aruoma OI. Free radicals and antioxidants in cardiovascular health and disease. Internet J Med Update 2006;1:1-17.
7. Valko M, Izakovic M , Mazur M, Rhodes CJ, Telser J. Role of oxygen radicals in DNA damage and cancer incidence. Mol Cell Biochem 2004;266: 37-56.
8. Valko M, Leibfritz D, Moncola J, Cronin MD, et al. Free radicals and antioxidants in normal physiological functions and human disease. Review. Int J Biochem Cell Biol. 2007;39:44-84.
9. Droge W. Free radicals in the physiological control of cell function. Review. Physiol Rev. 2002;82:47-95.
10. Willcox JK, Ash SL, Catignani GL. Antioxidants and prevention of chronic disease. Review. Crit Rev Food SciNutr 2004;44:275-95.
11. Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in health and disease. Physiol Rev 2007;87:315-424.
12. Genestra M. Oxyl radicals, redox-sensitive signalling cascades and antioxidants. Review. Cell Signal. 2007;19:1807-19.
13. Halliwell B. Biochemistry of oxidative stress. BiochemSoc Trans. 2007;35:1147-50.
14. Young I, Woodside J. Antioxidants in health and disease. J Clin Pathol.2001;54:176–86.
15. Mahajan A, Tandon VR. Antioxidants and rheumatoid arthritis. J Indian Rheumatol Ass. 2004;12:139-42.
16. Yu, B.P.Cellular defences against damage from reactive oxygen species. Biol Rev.1994; 74: 139-162.
17. Ray, G. and Husain, S.H.Oxidants, antioxidants and carcinogenesis. Indian J. Exp.Biol. 2002;40:1213-1232.
18. MacLean CH, Louie R, Leake B, etal.Quality of care for patients with rheumatoid arthritis, JAMA. 2000; 284: 984-992.
19. Yagi K. Lipid peroxides and human diseases. ChemPhys Lipids.1978; 45: 337-51.
20. Ravin, H.A . An improved colorimetric enzymatic assay for ceruloplasmin. J. Lab. Clin. Med.1961; 58: 161-168.
21. Roe JH, Kuther CA. Detection of ascorbic acid in whole blood and urine through the 2, 4, DNPH derivative of dehydroascorbic acid. J. Biol. Chem. 1943;147: 399-401.
22. Desai, F.D. Vitamin E analysis methods for animal tissues. In: Feischer S, Packer L, eds. Methods Enzymol, vol.105. Academic Press, New York .1984.138-145.
23. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: Biochemical roles as a component of glutathione peroxidase. Science. 1973; 179: 588-90.
24. Kakkar, P., Das, B. and Viswanathan, P.N. A modified spectrophotometric assay of superoxide dimutase. Indian J. Biochem. Bio.Phys. 1984; 21: 130-132.
25. Sinha, K.A . Colorimetric assay of catalase. Annal. Biochem. 1972; 47: 389-394.
26. Wintrobe MM, Landsberg JW. A Standardized technique for the blood sedimentation test. Am. J. Med. Sci. 1936; 189:102.
27. Kumagai S, Jikimoto T, Saegusa J. Pathological roles of oxidative stress in autoimmune diseases. RinshoByori. 2003;51(2):126-32.
28. GutteridgeJMC . Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin. Chem. 1995; 41: 1819-1828.
29. Kwon TW, Watts BM. Malondialdehyde in aqueous solution and its role as a measure oflipid oxidation in food. J Food Sci.1964; 29: 294-302.
30. ShaabaniY, Foroughi M, Rastmanesh R, Jamshidi A, Tajik N, Assadi O. Assessment of antioxidant nutrient intake and malondiadehyde plasma level in rheumatoid arthritis. Atherosclerosis J. 2009; 5: 1-5.
31. Ansari S, Jaiswal G. Oxidative status in rheumatoid arthritis–A biomedical study. Antiseptic. 2008;105:429-431.
32. Hassan MQ, Hadi RA, Al-Rawi ZS, Padron VA, Stohs SJ. The glutathione defence system in the pathogenesis of RA. J ApplToxicol. 2001;21(1):69–73.
33. Bae SC, Kim SJ, Sung MK. Inadequate antioxidant nutrient intake and altered plasma antioxidant status of rheumatoid arthritis patients. J Am CollNutr. 2003; 22: 311-315.
34. Sarban S, Kocyigit A, Yazar M, Isikan UE. Plasma total antioxidant capacity, lipid peroxidation, and erythrocyte antioxidant enzyme activities in patients with rheumatoid arthritis and osteoarthritis. ClinBiochem .2005; 38:981–6.
35. Mahajan M, Kaur S, Mahajan S, Kant R. Uric acid a better scavenger of free radicals than vitamin C in rheumatoid arthritis.Indian. J. Clin. Biochem. 2009; 24 (2): 205-207.
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Published
2013-10-31
How to Cite
Alam, R., Khan, S., & Salman, K. A. (2013). Evaluation of Antioxidants and Lipid Peroxidation Status in Rheumatoid Arthritis Patients: Assessment of Oxidative Stress in RA Patients. National Journal of Integrated Research in Medicine, 4(5), 1–5. https://doi.org/10.70284/njirm.v4i5.2219
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