Comparative Evaluation of Methods Used For Detection of Biofilm Production In Multidrug Resistant Bacteria
Comparative Evaluation of Methods Used For Detection of Biofilm Production
Keywords:
Biofilm, Multidrug resistant bacteria, Congo red agarAbstract
Background: Multidrug resistant organisms (MDROs) are defined as bacteria resistant to at least one agent in three or more antimicrobial classes. Infections due to these bacteria lead to prolonged antimicrobial therapy and increase morbidity and mortality in the patients admitted in G.B.Pant Hospital, New Delhi. These bacteria tend to attach to various medical devices and form biofilms. Biofilm production of MDR strains has not been evaluated so far in this set up. Objective: The study was conducted to detect and compare biofilm production of MDR bacteria isolated by three different methods of biofilm detection, Congo Red Agar (CRA) method, Tube Method (TM) and Tissue Culture Method (TCP). Methods: A total of 200 bacterial isolates (MDROs) from various clinical samples of patients suffering from hospital acquired infections were subjected to biofilm detection along with positive and negative controls by all three methods (TCP, TM, and CRA). Result: Of the 200 isolates (MDROs), 152 (76%) were biofilm producers i.e. strong 28 (14%), moderate 57(28.5%), weak 67 (33.5%) by TCP Method. By TM 166 (83%) were biofilm producers i.e strong 34 (17%), moderate 86 (43%) and weak 46 (23%). By CRA Method 126 (63%) isolates were biofilm producers and 74 were non-biofilm producers. False positive rate was 15% and 16.5% and false negative rate was 8% and 29.5% respectively by TM and CRA methods, taking in to consideration TCP as a Gold Standard method of biofilm detection.11 Conclusion: Tube Method has detected the highest number of biofilm producers followed by TCP and CRA. But Tube Method (TM) cannot be considered as most reliable method as the interpretation of the result depends upon the individual’s observations. False negativity was high in case of CRA method. So it is concluded that TCP is the most reliable quantitative method of screening of biofilm production of bacterial isolates in Laboratories. [Gyaneshwar T NJIRM 2017; 8(5):1-8]
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