Study of Asymptomatic Bacteriuria in Type 2 Diabetes Mellitus Patients
Study of Asymptomatic Bacteriuria in Type 2 Diabetes Mellitus Patients
Keywords:
Asymptomatic Bacteriuria, Escherichia coli, Leucocyturia,Type 2 diabetes mellitusAbstract
Background & Objectives: Diabetic subjects, especially women, show high prevalence of asymptomatic bacteriuria (ASB). The risk of urinary tract infection (UTI) is higher in diabetics compared to non-diabetics. The aetiology and the antibiotic resistance of uropathogens have been changing over the past years. Hence the present study was undertaken to evaluate the prevalence of asymptomatic bacteriuria (ASB) in patients with type 2 diabetes mellitus and to find out the antibiotic sensitivity pattern of bacterial isolates. Methods: Two hundred type 2 diabetics comprising males and females (aged between 30 - 80 years) who attended Maharashtra Institute of Medical Education & Research & BSTR hospital, Pune India were included in the study. Mid-stream urines were collected from patients aseptically into sterile wide mouth container and examined microscopically for leucocyturia, and were cultured using standard techniques on blood agar, Mac Conkey agar and incubated at 37°C aerobically for 24 h. Isolates were tested against separate antibiotics for gram negative and gram positive organisms by the disc diffusion method. Results: Significant bacteriuria was observed in 52% of urine samples (50 females and 54 males) . Bacteria isolated included Escherichia coli (31%), Klebsiella pneumoniae (13%),Citrobacter koseri(13%) ,Pseudomonas aeruginosa (10%), Proteus vulgaris (3%),Acinetobacter baumannii (2%) among GNB & Staphylococcus aureus (25%) among GPCs. E.coli showed maximum sensitivity to Nitrofurantoin, while few Klebsiella and Pseudomonas isolates were sensitive to Piperacillin-tazobactum. Acinetobacter baumannii showed high resistance to all antibiotics. Among all antibiotics,majority of isolates were resistant to Ampicillin-sulbactum. 50% Staphylococcus aureus isolates showed sensitivity to Nitrofurantoin. Leucocyturia was observed in 80% patients who showed ASB. Conclusion: Finding of leucocyturia can be a clue to the screening for ASB in diabetic patients, since ASB has been found to be a risk factor for developing symptomatic urinary tract infection in these patients. [Ketaki K NJIRM 2017; 8(3):12-16]
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